Avicenna J Med Biotech

arij002

Avicenna Journal of Medical Biotechnology

2008-2835 2008-4625

Avicenna Research Institute

ajmb259

The Effects of Berberine and Palmatine on Efflux Pumps Inhibition with Different Gene Patterns in Pseudomonas aeruginosa Isolated from Burn Infections

Aghayan

Seyed Sadjjad

Department of Biology, School of Basic Sciences, Golestan University, Gorgan, Iran

Kalalian Mogadam

Hamidreza

Department of Medical Biotechnology, Faculty of Advanced Technologies in Medicine, Golestan University of Medical Science, Gorgan, Iran

Fazli

Mozhgan

Genetics and Metabolism Research Group, Pasteur Institute of Iran, Tehran, Iran

Darban-Sarokhalil

Davood

Faculty of Medical Sciences, Shahrekord University of Medical Sciences, Shahrekord, Iran

Khoramrooz

Seyed Sajjad

Islamic Azad University Gorgan Branch, Gorgan, Iran

Jabalameli

Fereshteh

Cellular and Molecular Research Center, Taleghani Children Hospital, Gorgan University of Medical Sciences, Gorgan, Iran

Yaslianifard

Somayeh

Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Mirzaii

Mehdi

Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

9 1 2 7 14 1 2015 30 4 2016

Background: Related Multidrug Resistance (MDR) to efﬂux pumps is a signiﬁcant problem in treating infections caused by Pseudomonas aeruginosa (P. aeruginosa). Plant compounds have been identified as Pump Inhibitors (EPIs). In the current study, the potential effect of Berberine and Palmatine as EPIs were investigated on efflux pump inhibition through focusing on different gene patterns in P. aeruginosa isolated from burn infections. Methods: All isolates were collected and identified using the standard biochemical tests. Antimicrobial sensitivity was performed based on disk agar diffusion method for 12 antibiotics. MIC-MBC tests were also performed based on the broth microdilution method to detect synergistic relationship between ciprofloxacin, Berberine and Palmatine. Detection of mexA, mexB, mexC, mexD, mexE, mexF and mexX was conducted by PCR assay. Fisher's Exact test and Logistic Regression were used as statistical tools. Results: A total of 60 P. aeruginosa isolates were collected. The highest and lowest levels of resistance were found to be respectively against clindamycin and tigecycline. Comparing the MIC with MBC distribution, it was found that Berberine and Palmatine lower the MIC-MBC level of ciprofloxacin. The PCR results indicated that the highest frequency is about MexAB-OprM operon. The statistical analysis among different gene patterns of efflux pumps showed that there were no significant relationships between the effectiveness of Berberine and Palmatine (p>0.05). Conclusion: It can be speculated that Berberine and Palmatine both act as EPIs and can be used as auxiliary treatments with the purpose of increasing the effect of available antibiotics as well as decreasing the emergence of MDR bacteria. The efficiency of these combinations should be studied further under in vivo conditions to have a more comprehensive conclusion regarding this issue.