Avicenna J Med Biotech

arij002

Avicenna Journal of Medical Biotechnology

2008-2835 2008-4625

Avicenna Research Institute

ajmb10385

Cloning and Expression of B. mellitensis bp26 Gene in Lactococcus lactis as a Food Grade Vaccine

Azizpour Maghvan

Maryam

Jafari

Parvaneh

Bioinformatics and Computational Omics. LAB (BioCOOL), Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, Iran

Hosseini

Seyyed Davood

Department of Animal Science, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran

Behrozikhah

Ali mohammad

11 3 264 267 13 1 2018 8 4 2018

Background: Brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. Brucella melitensis (B. mellitensis) bp26 gene is a good candidate for brucellosis vaccine and investigations showed that Lactococcus lactis (L. lactis) with several positive characteristic are attractive for protein expression as a live delivery vectors. These fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive. Methods: B. mellitensis bp26 gene was cloned in food grade pNZ 8149 vector and expressed in L. lactis NZ 3900. Results: Results showed that we can produce a food-grade recombinant L. lactis producing the B. melitensis BP26 protein.  Conclusion: In this study, for Future evaluation about ability of L. lactis as a live delivery vector, a food-grade recombinant L. lactis producing the B. melitensis BP26 protein was produced.