<p>Background: The present study focused on the production of L-asparaginase using Solid State Fermentation (SSF) by <em>Myroides gitamensis</em>.&nbsp;<br /> Methods: Initially, five significant parameters (Carbon source; Nitrogen source, temperature, pH and incubation period) were identified that affect the production process of L-asparaginase using Classical One Factor at a Time (OFAT) optimization. An optimized L-asparaginase specific activity obtained by OFAT was recorded as 85.7 <em>IU</em>. Central Composite Design (CCD) was also employed successively to optimize the multiple parameters at a time and their results were compared.&nbsp;<br /> Results: Maximum L-asparaginase enzyme specific activity obtained by CCD method was 295.6 <em>IU</em> under the hold values of carbon source (wheat bran) 12 <em>g/L</em>, nitrogen source (yeast extract) 7 <em>g/L</em>, temperature 37^{<span style="font-size:10.8333px">o</span>}<em>C</em>, pH=7.5 and incubation period 47 <em>hr</em>. Upon validation, the obtained results proved that there was a good relation existing between the experimental and the predicted model (p&lt;0.05). L-asparaginase activity was enhanced in statistical method up to 3.4 folds compared to that of classical method.&nbsp;<br /> Conclusion: Utilization of wheat bran as a low cost carbon source in SSF for the production of L-asparaginase enzyme makes the process economical and in turn reduces the environmental pollution by biotransformation to commercially useful bio product.</p>