<p>Background: Disruption of the cholinergic neurotransmitter pathway which is important for cognition, memory and learning abilities has been reported in Alzheimer&rsquo;s Disease (AD) patients. The receptors involved include the Cholinergic Receptors Muscarinic (<em>CHRM</em>). <em>CHRM2</em> gene has been associated with intelligence, personality traits, substance dependence and depression. <em>CHRM3</em> has been found to heterodimerize with <em>CHRM2</em>.<br /> Methods: DNA samples from 240 AD patients with SNPs rs6962027 of <em>CHRM2</em> gene and rs7511970 of <em>CHRM3</em> gene were amplified using PCR and genotyped using Restriction Fragment Length Polymorphism (RFLP). Chi-squared test was done to check if the genes are in Hardy-Weinberg equilibrium.&nbsp;<br /> Results and Conclusion: Although the results did not show significant associations, these data denote plausible interaction between TT in SNP rs6962027 in <em>CHRM2 </em>gene and TT in SNP rs7511970 in CHRM3 gene affecting AD risk. SNP rs7511970 of <em>CHRM3</em> gene may also exert an influence on late-onset AD.</p>