Avicenna J Med Biotech

arij002

Avicenna Journal of Medical Biotechnology

2008-2835 2008-4625

Avicenna Research Institute

ajmb10343

Interleukin-2 and Interferon-Gamma Single Nucleotide Polymorphisms in Iranian Patients with Chronic Heart Failure

Mahmoudi

Mohammad Jafar

Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Clinical Biochemistry, Radiopharmacy Lab, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Harsini

Sara

Farhadi

Elham

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

Hedayat

Mona

Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

Taghvaei

Mohammad

Department of Biotechnology, College of Science, Tehran University, Tehran, Iran

Mahmoudi

Maryam

Department of Biology, Shahed University, Tehran, Iran

Sadr

Maryam

Department of Biology, Research Center for Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Esfahanian

Nilufar

Department of Biology, Shahed University, Tehran, Iran

Nematipour

Ebrahim

Department of Biology, Shahed University, Tehran, Iran

Nourijelyani

Keramat

Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Amirzargar

Ali Akbar

Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR , Tehran, IranDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences , Tehran, Iran

Rezaei

Nima

Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran , Tehran, Iran

10 3 173 177 29 8 2017 14 10 2017

Background: Inflammatory cytokines have been known to be associated with Chronic Heart Failure (CHF). Given the importance of cytokines in the context of the failing heart, the prevalence of Interleukin-2 (IL-2) and Interferon-gamma (IFN-γ) polymorphisms was studied in patients with CHF due to ischemic heart disease in a case-control study. Methods: Fifty-six Iranian patients with CHF were enrolled in this study as the case group and compared with 139 healthy subjects, using polymerase chain reaction with sequence-specific primers method, so as to determine the frequency of alleles, genotypes and haplotypes of IFN-γ (+874 A/T) and IL-2 (-330 G/T, +166 G/T) SNPs.  Results: The GG genotype at IL-2 -330 in patients with CHF was significantly overrepresented in comparison with the control group (p=0.013). Such a positive genotypic association was also observed for IL-2 +166/TT (p=0.022). Meanwhile, the GT genotype frequency at IL-2 -330/GT in the patient group was significantly lower than the one in healthy controls (p=0.049). No significant association was detected between the IFN-γ gene polymorphisms and individuals’ susceptibility to CHF. Conclusion: Certain genotypes in IL-2 gene were overrepresented in patients with CHF, which could render individuals more vulnerable to this disease.