<p>Background: Mutations in the coding region of the Chemokine Receptor 5 (<em>CCR5</em>) genes reduce or eliminate <em>CCR5</em> expression in immune cells and progression of HCV infection. This study aimed to investigate the role of this mutation in HCV infection in Iranian patients in comparison with healthy individuals.<br /> Methods: 100 HCV infected patients and 100 healthy individuals were randomly selected. The <em>CCR5&Delta;32</em> genotypes were determined using specific primers and PCR method.<br /> Results: The agarose gel electrophoresis showed a189-bp fragment from wild type for both alleles of <em>CCR5 </em>gene. The <em>CCR5-&Delta;32</em> allele was not found in any HCV infected and healthy subjects.<br /> Conclusion: The mutation in <em>CCR5</em> gene was not detected in any of the two groups; therefore, the role of<em> CCR5</em> gene expression in immune cells and progression of HCV infection needs to be studied in larger samples in our country.</p>