en
2008-2835
2008-4625
276
5669
11181
gregorian
>2020
>January-March
12
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online
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fulltext
en
32153740
Evaluation of the Bioactive Potential of Secondary Metabolites Produced by a New Marine Micrococcus Species Isolated from the Persian Gulf
<p><strong>Background</strong><strong>:</strong> In the present work, a newly isolated marine bacterium, <em>Micrococcus</em> sp. MP76, from coastal area of Persian Gulf around Bushehr province, Iran, was identified with the ability to produce bioactive compounds.</p>
<p><strong>Methods:</strong> The pigment production was optimized by changing carbon and nitrogen sources in bacterial growth media at 28<em>°</em><em>C</em> and 220 <em>rpm</em> for 5 days. Partial purification of the pigment was carried out using suitable solvents.</p>
<p><strong>Results:</strong> Maximum pigment extract was achieved (1.4 <em>g/l</em>) when cultured in the medi<em>um</em> containing 0.5% (v/v) molasses, 0.5% (w/v) peptone, 1% (w/v) sea salt, 0.01% (w/v) potassium phosphate, and 0.05% (w/v) yeast extract, pH=7.0. Antibacterial effect assessment of the extract against pathogenic bacteria revealed the MIC values in the range of 4.2-7.5 <em>mg/ml</em> depending on different pathogens. The pigment extracted from medium supplemented by molasses and ammonium sulfate had 81% radical scavenging activity, and its IC<sub>50</sub> value was 0.28 <em>mg/ml</em>.</p>
<p><strong>Conclusion:</strong> The newly isolated strain of <em>Micrococcus</em> genus from the Persian Gulf revealed a valuable source to access worth medicinal ingredients when cultured under optimized conditions.</p>
Antibacterial effect, Antioxidant activity, Bioactive compounds, Marine bacteria, Persian Gulf
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https://www.ajmb.org/En/Article.aspx?id=20420
https://www.ajmb.org/PDF/En/FullText/20420.pdf
Hamid RezaKarbalaei-HeidariInstitute of Biotechnology, Shiraz University, Shiraz, Iran31518
MozhdehPartovifarMolecular Biotechnology Lab., Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran31571
MinaMemarpoor-YazdiMolecular Biotechnology Lab., Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran31572