Avicenna J Med Biotech arij002 Avicenna Journal of Medical Biotechnology 2008-2835 2008-4625 Avicenna Research Institute ajmb10385 Cloning and Expression of <i>B. mellitensis bp26</i> Gene in <i>Lactococcus lactis</i> as a Food Grade Vaccine Azizpour MaghvanMaryamJafariParvanehBioinformatics and Computational Omics. LAB (BioCOOL), Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, IranHosseiniSeyyed DavoodDepartment of Animal Science, Research Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, IranBehrozikhahAli mohammad 11 3 264 267 13 1 2018 8 4 2018

<p>Background: Brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. <em>Brucella melitensis (B. mellitensis) bp26</em> gene is a good candidate for brucellosis vaccine and investigations showed that<em> Lactococcus lactis (L. lactis)</em> with several positive characteristic are attractive for protein expression as a live delivery vectors. These fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive.<br /> Methods: <em>B. mellitensis</em> <em>bp26</em> gene was cloned in food grade pNZ 8149 vector and expressed in <em>L. lactis</em> NZ 3900.<br /> Results: Results showed that we can produce a food-grade recombinant <em>L. lactis </em>producing the <em>B. melitensis </em>BP26 protein.&nbsp;<br /> Conclusion: In this study, for Future evaluation about ability of L. lactis as a live delivery vector, a food-grade recombinant <em>L. lactis</em> producing the <em>B. melitensis</em> BP26 protein was produced.&nbsp;</p>